Figure 4.

Dechlorination and extracellular cobamide production in Dehalococcoides mccartyi strain BAV1/Methanosarcina barkeri strain Fusaro and strain BAV1/Sporomusa ovata co-cultures. (a) Strain BAV1/Methanosarcina barkeri strain Fusaro co-culture amended with 25 µg l⁻¹ CN-Cbl (positive control); (b) strain BAV1/strain Fusaro co-culture without CN-Cbl addition; (c) strain BAV1 culture without CN-Cbl (negative control); (d) Strain BAV1/Sporomusa ovata co-culture amended with 25 µg l⁻¹ CN-Cbl (positive control); (e) strain BAV1/Sporomusa ovata co-culture without CN-Cbl addition; (f) strain BAV1/Sporomusa sp. strain KB-1 co-culture without CN-Cbl addition. Filled triangle, cis-DCE; open inverted triangle, vinyl chloride; filled square, ethene; open circle, acetate; cross symbol, cobamide(s) reported as CN-Cbl equivalents. Error bars represent the standard deviations of triplicate samples and are not shown if they are smaller than the symbols.