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. 2013 Apr 19;368(1616):20120321. doi: 10.1098/rstb.2012.0321

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© 2013 The Author(s) Published by the Royal Society. All rights reserved.

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Figure 1. — Cell concentration of strain CBDB1 after cultivation in the standard defined medium lacking specific cofactors. Bars show mean values of five parallel cultures, and standard deviations are indicated. The treatments depicted in the figures are as follows: V0, negative control without cofactors; V7, positive control containing the seven vitamins cyanocobalamin (B₁₂), biotin (BIO), thiamine (THI), p-aminobenzoate (PABA), pantothenate (PAN), nicotinate (NIC) and pyridoxine hydrochloride (PDX); V6, V7 without B₁₂. The symbol minus or plus preceding a vitamin abbreviation indicates that a specific cofactor was omitted or added, respectively. DCC, dicyanocobinamide; Co(2+), cobalt(II)chloride; DMB, dimethylbenzimidazole. (a) Vitamin requirements for pyridoxal-5-phosphate, nicotinate, pantothenate and cyanocobalamin; (b) vitamin requirements for thiamine and biotin; (c) cobalamin precursors; (d) effect of biotin removal with avidin.