Figure 2.

Folate biosynthesis in Dehalococcoides as inferred from cultivation, genome annotation, nLC–MS/MS and inhibition studies. Boxes show gene name, E.C. number of the encoded enzyme and a short name of the enzyme. PABA, p-aminobenzoate; GTPCH I, guanosine triphosphate cyclohydrolase I; PTPS-III, 6-pyruvoyltetrahydropterin synthase, type III [60]; HPPK, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase; DHPS, dihydropteroate synthase; THFS, dihydrofolate synthase/tetrahydrofolate synthase; DHFR, dihydrofolate reductase. Only FolE (CbdbA1120) was found in one of 30 nLC–MS/MS data sets from cultures of strain CBDB1.