Figure 6.

Evaluation of biochemical and metabolomic parameters in P. pavonaceae cells growing exponentially on MMY medium containing either 30 mM d-glucose or citrate (i.e. glycolytic and gluconeogenic metabolic regimes) under control conditions (nil) and in the presence of 0.5 mM 1,3-dichloroprop-1-ene (1,3-DCP). (a) In vitro quantification of the specific (Sp) d-glucose-6-P dehydrogenase activity. Note that the superoxide-generating naphtoquinone menadione was added at 0.15 mM to some cultures to impose oxidative stress conditions. (b) Determination of the intracellular d-glucose-6-P concentration. Appropriate aliquots of clarified cell-free extracts were boiled and centrifuged, and supernatants were spectrophotometrically analysed for d-glucose-6-P as detailed in §2. Each bar represents the mean value of the corresponding parameter ± s.d. of duplicate measurements from at least three independent experiments. In both cases, the asterisk marks denote significant differences in the corresponding parameter at either the 95% level (single asterisk) or the 99% level (double asterisks) when compared with the control culture (without additives), as evaluated with ANOVA.