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Estimation of system parameters was achieved by fitting experimental results with model solutions using the Residual Sum of Squares method. The mean of the experimental data and its corresponding best-fit curve is represented here. (A) Acellular gels were used to characterize the diffusion coefficient D (r2=0.99) and (B) cellular gels were used to characterize the Michaelis-Menten parameters Vmax and Km (r2=0.93). Color images available online at www.liebertpub.com/tea
