Table 1.
Counting of daily detached, trypan blue stained and viable LLC-PK1 cells during the GM exposure and SFN treatment.
| CT | SFN | GM | SFN + GM | |
|---|---|---|---|---|
| DC/well 24 h | 1,483 ± 367 | 483 ± 60 | 5,487 ± 247* | 1,113 ± 567# |
| DC/well 48 h | 1,633 ± 109 | 1,117 ± 469 | 8,033 ± 324* | 4,250 ± 375# |
| DC/well 72 h | 2,867 ± 192 | 2,883 ± 259 | 8,017 ± 760ε | 4,733 ± 838Δ |
| TBS cells/well (attached after GM exposure) | 16,517 ± 3,219 | 23,733 ± 8,020 | 31,800 ± 3,318 | 25,433 ± 829 |
| Total death cells/well | 22,500 ± 3,399 | 28217 ± 8,366 | 53,337 ± 3,714ε | 35,550 ± 1,422 |
| Viable cells/well | 106,450 ± 4,976 | 94,317 ± 5,874 | 26,883 ± 4,681* | 60,400 ± 5,765Δ |
CT: cells without treatment. SFN: cells incubated in 5 µM SFN. GM: cells in 8 mM GM. SFN + GM: cells coincubated in 5 µM SFN and 8 mM GM. DC: detached cells. TBS: trypan blue stained. Total death cells = DC 24 h + DC 48 h + DC 72 h + TBS cells. Cells attached after GM exposure include TBS cells + viable cells. Data are mean ± SEM, n = 3. *P < 0.001 versus CT; # P < 0.001 versus GM; ε P < 0.01 versus CT; Δ P < 0.05 versus GM.