Table 1.
Cytotoxic activity, (∗) expressed as percent of affected sea urchin (Lytechinus variegatus) embryos, of methanol extract (MeOH) and hexane, chloroform (CHCl3), ethyl acetate (EtOA), and methanol (MeOH) fractions at 32 µg mL−1 (see text) from samples (∗∗) of Brazilian propolis. No effect was observed in tests using ethanol (control).
| Extract/fractions (µg/mL) | Samples | |||||
|---|---|---|---|---|---|---|
| A | B | C | D | E | F | |
| MeOH extract | 98.5 ± 2.1 | 97.3 ± 2.1 | 100 ± 0.0 | 99.3 ± 0.6 | 97.7 ± 3.2 | 97.3 ± 1.5 |
| Fractions | ||||||
| Hexane | 98.5 ± 2.1 | 64.7 ± 7.5 | 27.5 ± 0.7 | 5.0 ± 2.1 | 4.3 ± 2.1 | 6.3 ± 1.5 |
| CHCl3 | 83.0 ± 1.4 | 83.3 ± 7.5 | 64.0 ± 4.2 | 92.5 ± 0.7 | 2.0 ± 1.0 | 15.0 ± 1.4 |
| EtOAc | 95.5 ± 2.1 | 98.0 ± 2.0 | 100 ± 0.0 | 100 ± 0.0 | 99.7 ± 0.6 | 100 ± 0.0 |
| MeOH | 3.5 ± 2.1 | 2.3 ± 1.2 | 3.3 ± 0.6 | 3.5 ± 0.7 | 4.7 ± 2.5 | 1.7 ± 1.2 |
*Mean ± s.d.; **A–D: samples from Minas Gerais, E and F: samples from Paraná state.