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The effect of ESL (a) and EE (b) on insulin signaling in skeletal muscles. Analyses of insulin-induced phosphorylation of IRβ, AKT, and P70S6K were performed by western blot. Following an overnight fast, mice were either sacrificed for or injected with 5 U/kg of insulin. Five minutes after injection, muscle tissues were collected and total protein was analyzed. The phosphorylation site in each protein is indicated.
