Fig. 3.

Cardiolipin dependence by the ATP synthase for oligomycin sensitive ATPase activity. a The ATP synthase was purified in the absence of lipids, cardiolipin (BH CL) was bound (200 molar excess) and assayed in buffer containing 0.006% DDM (I) in the absence of additional added lipid and in buffer containing 0.05% DDM and 0.03% lecithin (II). b The ATP synthase was purified in the absence of lipids and assayed in the presence and absence of oligomycin (5 μg) in buffer containing 0.005% DDM with the indicated amount (molar excess) of TM CL