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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: J Neurochem. 2008 Jul 1;106(1):464–474. doi: 10.1111/j.1471-4159.2008.05398.x

Fig. 4.

Fig. 4

PINK1 interacts with Hsp90. (a) Stably transfected HeLa cells were treated for up to 4 h with 1 μM 17-AAG, an inhibitor of Hsp90 activity. 17-AAG treatment significantly decreased PINK1 Δ2 levels. (b) Quantification of each PINK1 isoform level from three independent 17-AAG experiments as represented in (a). Results are expressed as mean ± SEM. While expression of PINK1 Δ2 continued to decrease over time, level of PINK1 FL and Δ1 did not decrease after 30 min of 17-AAG treatment. (c) FLAG co-immunoprecipitation in HeLa cells transiently transfected with PINK1-flag. Cells were treated with 1 μM 17-AAG for 30 min; 1 μM MG132 was added to prevent 17-AAG-induced degradation. Lysates were immunoprecipitated with anti-flag antibodies and immunoblotted for flag or Hsp90β. Total lysates were used to detect Hsp90β and PINK1-flag level by western blot. 17-AAG led to a dissociation of PINK1-flag from Hsp90β.