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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Hematol. 2012 Sep 11;41(1):91–101.e4. doi: 10.1016/j.exphem.2012.09.001

Figure 1.

Figure 1

Increased expression of β-sGC, PKG-I, PKA-C, VASP, and MENA during differentiation of mouse FLC into MKs. (A) FLC and MKs separated from non-MK cells after 2 and 4 days in culture and mouse platelets (Plt) were analyzed for expression levels of endothelial NOS (eNOS), β-sGC, PKG-I, PKA-C (catalytic subunit), MENA, VASP, and phospho-VASP (Ser157, Ser239) by Western blotting. Human umbilical vein endothelial cells were used as a control for eNOS and mouse brain lysate as a control for MENA expression. GAPDH was used as a loading control. Blots representative of four independent experiments are shown. (B) Proplatelets contain PKG-I, VASP, and MENA. Immunofluorescence images of MKs with proplatelets stained for PKG-I, VASP, MENA, or β-tubulin. The images are representative of four independent experiments. (C) Colocalization of VASP phosphoforms in proplatelets. Immunofluorescence images of proplatelets stained with mouse anti–phospho-VASP Ser157 and rabbit anti–phospho-VASP Ser239 representative of three independent experiments are shown. Scale bar: 5 μm.