. Author manuscript; available in PMC: 2013 Apr 29.

Published in final edited form as: J Steroid Biochem Mol Biol. 2004 Jan;88(1):101–110. doi: 10.1016/j.jsbmb.2003.11.002

Table 1.

Effect of high glucose on the interaction of growth factors with cell proliferation and CK specific activity in human vascular cells

³[H] thymidine incorporation into DNA (% change)
	E304		VSMC
	Low glucose	High glucose	Low glucose	High glucose
C	0 ± 13	0 ± 19	0 ± 17	0 ± 14
IGF1	100 ± 20	110 ± 15^*	340 ± 25^***	248 ± 30^***,^#
PDGF	148 ± 10^**	186 ± 15^**,^#	275 ± 30^**	227 ± 20^***
Creatine kinase specific activity (% change)
C	0 ±15	0 ± 9	0 ± 3	0 ± 19
IGF1	68 ± 12^*	88 ± 15^*	68 ± 8^*	61 ± 23^*
PDGF	155 ± 12^**	133 ± 35^**	58 ± 8^*	110 ± 20^**,^##

Effects of IGF1 (5 ng/ml) and PDGF (50 ng/ml) in the presence or absence of high glucose on ³[H] thymidine incorporation and on creatine kinase specific activity in VSMC and in E304 cells. Results are means±S.E.M. of eight incubates from two experiments and are expressed as percent change of ³[H] thymidine incorporation or enzyme activity in hormone-treated and control cells.

P < 0.05.

^**

P < 0.01.

^***

P < 0.001 for the comparison with control values at either low or high glucose.

P < 0.05.

^##

P < 0.01 for the comparison of values of stimulation at low and high glucose. High glucose stimulated DNA in VSMC by 43 ± 14% and in E304 by 64 ± 9% and CK in VSMC by 375 ± 19% and by 38 ± 9% in E304. The statistical analysis was done by ANOVA.