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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 5;1833(6):1489–1497. doi: 10.1016/j.bbamcr.2013.02.026

Figure 4. DDX3 regulates p53 accumulation after DNA damage.

Figure 4

Cytosolic (left panel) and nuclear (right panel) fractions were prepared from MCF-7 wild-type or DDX3 knockdown cells after treatment with 10 μM camptothecin for 4 hr, followed by measurements of p53 and DDX3 levels. β-tubulin was used as a loading control for cytosolic fractions, and CREB for nuclear fractions. Experiments were repeated at least two times with similar results.