Fig. 2.

Gene expression in rice gametes and zygotes. (A) Venn diagram of gene expression overlap in sperm cells, egg cells, and zygotes. Numbers indicate genes with significant t-tests (P <0.05) in each cell type. (B) Venn diagram of genes with substantially higher (>10-fold) expression levels in sperm versus egg cells and genes whose expression was clearly suppressed in whole plantlets. Data for whole plantlets were from Ohnishi et al. (2011). (C) Transcript levels of seven genes enriched in egg or sperm cells and of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in somatic tissues and gametes were monitored by quantitative PCR and normalized to ubiquitin mRNA. The numbers at the bars indicate values of relative mRNA levels. See Supplementary Table S1 at JXB online for primer sequences.