Fig. 6.

(A) Tobacco BY-2 cells co-transformed with (i) AtWEE1–YFP^C in the vector pSPYCE and AtSKIP1–YFP^N in the vector pSPYNE; (ii) AtBZIP63 in both pSPYCE and pSPYNE (positive control); and (iii) AtWEE1–YFP^C and AtBZIP63–YFP^N (negative control); under UV light (left), white light (right), and the two merged (centre). Blue colouring indicates a positive interaction between the two proteins (representative images). (B) Mean nuclear fluorescence frequency (%; ±SE, n=3) in each cell cycle phase in cells from the following transgenic BY-2 lines: AtWEE1–GFP line # 4 (GFP–WEE1) and AtWEE1–YFP^C/AtSKIP1–YFP^N, line # 6 (WEE1–C/SKIP1-N6).