Genetic characterization of TaSnRK2.3 in wheat. (A–C) Chromosome identification of TaSnRK2.3-A (A), TaSnRK2.3-B (B), and TaSnRK2.3-D (C) in common wheat. AA, T. urartu; SS, A. speltoides; DD, A. tauschii; AB, T. dicoccoide and T. dicoccum; ABD, T. aestvium; NT, nulli-tetrasomic lines of Chinese Spring; M, 200bp DNA ladder. (D) SNP of TaSnRK2.3-B between the two parents of the DH population. A SNP (T→C) occurring at 819bp of the B genomic allele of Lumai 14 resulted in the absence of a cutting site for TaqI. (This part is available in colour at JXB online.) (E) A cleaved amplified polymorphic sequence (CAPS) marker was developed with restriction enzyme TaqI. L, before digestion; R, after digestion with TaqI; H10, Hanxuan 10; L14, Lumai 14. (F) The CAPS marker was detected in species with A and/or B genomes. (G) Cleaved amplified sequence polymorphisms identified in the DH population. 1–27, Lines of the DH population, followed by the two parents. (H) The B genomic allele TaSnRK2.3-B was mapped on chromosome 1B flanked by wmc156 and P3346-183.