Fig. 7.
TaSnRK2.3 transgenics have stronger K+-retaining and Na+-extruding capabilities relative to WT Arabidopsis. Two transgenics had lower K+ ion efflux rates (A) and higher Na+ ion extruding rates (B) than WT plants. For K+ ion flux rate measurement, the Arabidopsis seedlings were pre-incubated in buffer (0.5mM KCl, 0.1mM MgCl2, 0.1mM CaCl2, 0.2mM Na2SO4, and 0.3mM MES, pH 6.0) for 30min and assayed in the same buffer containing 100mM NaCl. For Na+ ion efflux rate measurement, the Arabidopsis seedlings were pre-treated on MS medium supplemented with 100mM NaCl for 24h. Five plants were measured for each line, and the values are means ±SE (n= 5).