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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Cell Res. 2012 Sep 24;319(1):1–11. doi: 10.1016/j.yexcr.2012.09.003

Fig. 6.

Fig. 6

VCP Y805 is the sole tyrosine phosphorylated in ES cells. TC32 cells were transiently transfected with either GFP expressing control vector, or various VCP expression vectors (GFP-VCP-WT, GFP-VCP-YF, or GFP-VCP-YE) using electroporation. Cells were treated with 300 µM pervanadate for 30 min, lysed and subsequently subjected to immunoprecipitation using antibodies against GFP. The top panel is the immunoblot with antibodies against pY and the bottom blot is for GFP. A non-specific band (n.s.) was marked with the small arrow.