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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Cell Rep. 2012 Aug 16;2(2):372–385. doi: 10.1016/j.celrep.2012.07.009

Figure 1. Ubp10 Is Necessary for Optimal Cell Growth and Localizes to the Nucleolus.

Figure 1

(A) Plate growth of a parent strain compared to ubp10Δ, sir2Δ, sir3Δ, sir4Δ, ubp10Δ94-250, and ubp10C371S strains. Tenfold serial dilutions of cells were spotted on rich medium and incubated at 30°C for 3 days.

(B) Cell-cycle profiles for asynchronous cultures of strains from (A) were examined by propidium iodide staining and flow cytometry. Proportions of cells in G1 versus G2 phases are presented.

(C–E) Fluorescence microscopy of exponentially growing cells coexpressing Nop58-dsRed and either Sir4-GFP (C), Ubp10-GFP (D), or Sir2-GFP (E). Nop58-dsRed marks the nucleolus and DAPI staining marks chromatin.

(F) Spot tests of parent, ubp10Δ, or sir2Δ cells containing the rDNA silencing reporter RDN1::URA3. Cells were spotted in 10-fold serial dilutions onto media containing uracil to assess spotting efficiency or without uracil to measure rDNA silencing. Plates were incubated at 30°C for 3 days.