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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Sep 27;86(3):645–660. doi: 10.1111/mmi.12006

Fig. 2.

Fig. 2

Combined assays of the autokinase, phosphoryltransfer, and phosphatase activities mediated by truncated WalK+ or WalKT222A. Reactions were performed at 25°C in reaction mixtures containing Mg2+ or Ca2+ buffer as described in Experimental procedures. Autophosphorylation reactions containing 2.0 μM of purified (A) (N)-Sumo-ΔN35-WalK+ or (B) (N)-Sumo-ΔN35-WalKT222A protein proceeded for 30 min before (N)-His-WalR (final concn = 6.6 μM) was added without removal of ATP (t=0). The experiment was repeated >3 times, and representative phosphorimages of time courses are shown.