Fig. 6.

Dephosphorylation of WalR~P by WalK and WalK^T222A in combined assays containing physiologically relevant molar ratios of WalK sensor kinase to WalR response regulator (1:6.7) (Wayne et al., 2010). Combined assays were performed in buffers containing Mg²⁺ or Ca²⁺ ion as described in Experimental procedures, and amounts of WalR~P remaining after 30 min of incubation are shown. Similar trends were observed after 1 min and 60 min incubations (Fig. S13). Means with standard errors are indicated from 3 independent experiments.