Table 1.
Changes in relative transcript amounts in strain IU1896 (ΔwalK) compared to IU1781 (walK+) grown exponentially in BHI brotha
| Gene tag | Gene descriptionb | Fold changes |
Bayesian P value |
|---|---|---|---|
| Decreased relative expression | |||
| spd_0104 | LysM domain-containing protein | −3.1 | 8.6E-07 |
| spd_0391 | Hypothetical protein | −1.9 | 2.5E-05 |
| spd_0392 | Hypothetical protein | −1.8 | 5.2E-05 |
| spd_0447 |
glnR; transcriptional repressor of the glutamine synthetase gene |
−1.7 | 4.3E-04 |
| spd_0451 | hsdS; type I restriction enzyme | −2.5 | 1.5E-06 |
| spd_0703 | Hypothetical protein, putative SEDS protein | −1.9 | 3.0E-06 |
| spd_0853 | lytB, N-acetylglucosaminidase | −1.6 | 6.9E-04 |
| spd_1824 | Putative transmembrane protein with conserved FtsX superfamily domain |
−1.6 | 1.4E-04 |
| spd_1870 |
pcp-truncation; pyrrolidone carboxyl
peptidase, truncation |
−1.7 | 4.8E-05 |
| spd_1871 | Conserved hypothetical protein | −2.0 | 5.4E-06 |
| spd_1872 |
marR; transcriptional regulator - MarR
family |
−2.0 | 5.6E-05 |
| spd_1874 |
LysM domain-containing protein, putative
N-acetylmuramidase |
−4.4 | 2.7E-07 |
| spd_2043 | pcsB, putative peptidoglycan hydrolase | −2.6 | 1.6E-06 |
| Increased relative expression | |||
| spd_0094 | Hypothetical protein | 1.6 | 4.7E-04 |
| spd_0453 |
hsdS; type I site-specific deoxyribonuclease chain S |
2.8 | 6.0E-07 |
a
Strain construction, growth, and microarray analysis are described in Experimental procedures. RNA was prepared from exponential cultures grown to OD620 ≈ 0.2. Fold changes and Bayesian P-values are based on 3 independent biological replicates, including a dye swap. Cutoffs for this table were 1.6-fold and P<0.001. A complete data set is deposited in GEO (accession GSE19752).
b
Members of the WalRKSpn regulon are shown in bold type.