Figure 1.

Nuclear Transport Kinetics. CHO cultured cell monolayers were treated with serum-free medium containing 5 μg/mL ¹²⁵I-labeled peptides for the indicated time points at 37 °C. (A) The amount of ¹²⁵I-MAP internalized by endocytosis (solid line, primary axis) and accumulated in the nucleus (dashed line, secondary axis) was determined as described in the Materials and Methods section. Data are represented as the average of duplicate sample measurements and error bars indicate the standard error. (B) Treated cells were detached following incubation with trypsin and the nuclear accumulation was determined as described in the Materials and Methods section. The bars represent the average of triplicate sample measurements, the errors bars represent the standard deviation, and bars marked with an asterisk indicates a statistically significant difference (p < 0.05) in results from two different timepoints.