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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Mol Pharm. 2009 Mar-Apr;6(2):337–344. doi: 10.1021/mp800239p

Figure 5.

Figure 5

Release of Degradation Products in CHO cells. To determine the effect of ammonium chloride and chloroquine (CQ) on the release of degradation products following treatment with MAP, YG(K)9, or YG(R)9, cultured CHO cell monolayers were treated with serum-free medium containing 5 μg/mL 125I-labeled peptide for 1 hour at 37 °C in the absence (black bars) or presence of 50 mM NH4Cl (gray bars) or 200 μM chloroquine (open bars). The cell monolayers were then extensively washed, and fresh serum-free medium in the absence or presence of endocytosis inhibitors was added and the cells were incubated for an additional hour at 37 °C. Data are represented as a % Remaining in Cell ([amount of 125I-labeled peptide released to chase medium/total internalized]*100%).