Table 1.
For the reproducibility experiment with the analysis of ten mice, a subset of metabolite identifications are provided in the extraction of ~ 20 mg of heart tissue in 1200 μl chloroform and 800 μl methanol:water (1:1 by volume). Metabolite signals for the determination of quantitative precision (RSD) were calculated after signal deconvolution by PARAFAC.
| Metabolite | Column 1 Retention Time (s) | Column 2 Retention Time (s) | Quantitative Precision Detected Signal (RSD) |
|---|---|---|---|
| lactate | 481.5 | 0.41 | 21 % |
| glucose | 1278 | 0.21 | 40 % |
| fumarate | 778.5 | 0.69 | 15 % |
| malate | 922.5 | 0.48 | 19 % |
| succinate | 711 | 0.90 | 47 % |
| succinyl-CoA | 747 | 0.62 | 39 % |
| myo-inositol | 1422 | 0.28 | 26 % |
| coenzyme A | 633 | 1.20 | 11 % |
| glycerol | 633 | 0.21 | 26 % |
| glycerol-3-phosphate | 1084.5 | 0.77 | 25 % |
| glucose-1-phosphate | 1194 | 0.20 | 27 % |
| glutamate | 877 | 1.41 | 26 % |
| citric acid | 1130 | 0.47 | 24 % |
| serine | 615 | 0.71 | 27% |
| thiamine diphosphate | 747 | 0.71 | 14% |
| nicotinamide adenine dinucleotide | 1281 | 0.85 | 39% |
| uracil | 699 | 0.40 | 24% |