Figure 2.

The recorded channel is a Cx43 hemichannel. A, Fast-gating openings of Cx43 hemichannels in cell-attached patches from a Cx43-expressing C6 cell (Cx43⁺), a control Cx43-deficient cell (Cx43⁻) that does not express Cx43, a Cx43-eGFP-expressing C6 cell (Cx43-GFP), and a truncated Cx43-expressing C6 cell (Cx43-M257). B, The number of Cx43 hemichannels per cell-attached patch. C, Twenty-six picosiemen openings of Cx 43 hemichannels in inside-out patches from Cx43⁺, Cx43⁻, Cx43-eGFP, and Cx43-M257 cells. D, The mean number of 26 pS Cx43 hemichannels per inside-out patch. **p < 0.01 compared with Cx43⁺, Student's t test. E, Immunolabeling against Cx43 (white) in Cx43⁺, Cx43⁻, and Cx43-M257 C6 cells and immunolabeling against eGFP in Cx43-eGFP C6 cells (Cx43-GFP). Scale bars, 10 μm. Error bars indicate SEM.