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. 2012 Jan 13;22(4):547–561. doi: 10.1111/j.1750-3639.2011.00557.x

Figure 2.

Figure 2

A18 Co: Laminar distribution of major extracellular matrix components in area 18 of human control brain. Two neuronal markers [neuronal nuclear protein (NeuN) and parvalbumin (Parv)] are shown to indicate the basic laminar profile. Wisteria floribunda lectin (WFA) indicates many perineuronal nets in layer III and V. Low numbers of perineuronal nets can be seen in layer VI whereas layer I, II and IV are virtually devoid of WFA‐stained nets. Compared with WFA staining, pan‐aggrecan antibody HAG (Agg) detects more perineuronal nets, indicating heterogeneity in aggrecan glycosylation. This becomes evident in layer II and VI containing Agg positive nets but devoid of WFA‐stained perineuronal nets. Brevican antibody B50 (Brev) reveals dot‐like matrix structures in a clearly different laminar pattern. Immunoreactivity is mainly restricted to layers IIIb, IV, lower V. The open circle marks the lower edge of layer IV. Scale bar = 200 µm.