TABLE 1:
Cells harvested at t = 7.5 h | |||
---|---|---|---|
3MB-PP1 added at t (h) | Abnormal DNA states? | Bilobe duplication? | FAZ duplication? |
0 | Yes | Impaired | Impaired |
3.5 | No | Normal | Partial |
5.5 | No | Normal | Normal |
Cells synchronized by elutriation were treated with 3MB-PP1 after 0, 3.5, or 5 h in culture. The cells were then fixed and stained with DAPI to evaluate their DNA state, along with antibodies against the FAZ or bilobe to assess the status of these structures.