FIGURE 1:

PRA and PRB differentially enhance migration of MDA-MB-231 breast cancer cells. (A) MDA-iPRAB cells were incubated with vehicle (PR−), RSL1 (inducing PRA) or Dox (inducing PRB), or both (inducing PRA and PRB) for 24 h. Immunoblot analysis was performed from whole-cell extracts using anti-PR antibody recognizing both PR isoforms and anti-tubulin antibody for loading control. (B) MDA-iPRAB cells were induced as in A for 24 h and treated by mitomycin C for 1 h. At zero time point wound-healing repair assays were performed in the presence of either 10⁻⁸ M R5020 or vehicle for various time until 24 h. Photographs of each wounded area were taken at regular time and distance intervals (left). Results from three independent experiments are presented (right) as the random distance covered by cells after 10 h treatment (mean ± SEM, n = 3). Statistical analyses were done using Mann–Whitney tests (stars).