FIGURE 4:

PRA and PRB differentially affect regulation of FAK activity. (A) MDA-iPRAB cells were grown in the presence of Dox (PRB), RSL1 (PRA), or vehicle (PR−) for 24 h and analyzed by Western blot using alternately anti-FAK^Y397p, anti-FAK^total, and anti-tubulin antibodies as described in Materials and Methods. PR isoforms were analyzed on a separated gel using anti-PR antibody. A representative immunoblot is shown, and FAK^Y397p, FAK^total, and FAK^Y397p/FAK^total ratio were quantified and normalized to tubulin from three independent experiments. The corresponding graphs show fold change of values obtained in PR− cells (means ± SEM, Mann–Whitney statistical test). (B) Cells were induced as in A and then treated or not by 10⁻⁸ M R5020 for the indicated times (minutes). Cell lysates were analyzed as in A, and data are presented as fold change of values obtained for the corresponding PR isoform at zero time point.