Figure 7:

(A) Time range required (in seconds) for the irradiated pole to start to move toward the equator after laser microbeam irradiation during metaphase in PtK2 cells. (B) Distance of the irradiated and unirradiated poles from the equator in micrometers vs. time in minutes. The vertical line represents the time of the irradiation. The pole on the irradiated side moved toward the equator first soon after the irradiation. (C) PtK2 irradiated cell illustrating the position of the trap (red square) at the spindle pole and the line cut across the spindle. Bar, 10 μm. (D) Distance of the irradiated and unirradiated poles from the equator in micrometers vs. time in minutes. The vertical lines represent the time of the irradiation when the trap was turned on and when the trap was turned off. A trapping power of 7.8 mW was applied to the spindle pole of the irradiated metaphase half-spindle. When the trap was applied, the irradiated pole and the unirradiated pole did not move toward the equator.