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. 2013 May 1;24(9):1396–1410. doi: 10.1091/mbc.E13-01-0065

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© 2013 Cheon et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — The distribution and organization of hStim1-YFP and hOrai1-mRFP change during oocyte maturation. (A–D) The distribution of hStim1-YFP was examined using confocal microscopy from images taken at the equatorial plane of live hStim1-YFP–expressing oocytes. The number of oocytes examined at each stage is shown at the bottom of each representative image. hStim1-YFP displayed two distinct patterns of organization in all stages of maturation except the GV stage; the most representative configurations are shown, along with the proportion of oocytes/eggs exhibiting the particular pattern. (E, F) The distribution of hOrai1 was examined as in the foregoing in GV and MII oocytes expressing hOrai1-mRFP. Intensity profiles of the line scans drawn in oocytes and eggs are shown below E, and a bar graph displaying the relative intensity of Orai1 signal between PM and cytoplasm is shown below F. Scale bar, 10 μm.