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. 2013 May 1;24(9):1396–1410. doi: 10.1091/mbc.E13-01-0065

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© 2013 Cheon et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Overexpression of SOCE components alters Ca²⁺ homeostasis. (A) Expression of hStim1-YFP+hOrai1-mRFP increased Ca²⁺ influx in both GV and MII cells after TG and CaCl₂ add-back, although the increase was greater and more prolonged in GV oocytes. Expression of hStim1-YFP also increased Ca²⁺ influx in both GV and MII stages but to a lesser extent, whereas hOrai1 had no effect. (B) Expression of hStim1 or hStim1+hOrai1 differentially increased basal [Ca²⁺]_i during maturation. Baseline [Ca²⁺]_i traces in control (top, left), hStim1-YFP–expressing (top, right), hStim1-YFP+hOrai1-mRFP–expressing (bottom, left), and D76A hStim1-YFP+ hOrai1-mRFP–expressing (bottom, right) oocytes. Insets in each treatment depict a magnified version of the y-axis at the 0.1 mark so that minor differences in basal [Ca²⁺]_i between GV and MII stages can be appreciated for some of the treatments.