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. 2013 May 1;24(9):1396–1410. doi: 10.1091/mbc.E13-01-0065

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© 2013 Cheon et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Changes in Ca²⁺ homeostasis affect resumption of meiosis and oocyte maturation. (A) Control, sham-injected, and oocytes expressing hStim1-YFP, hStim1-YFP+hOrai1-mRFP, or D76A Stim1-YFP+hOrai1-mRFP were in vitro matured and their maturation rates assessed. Expression of any mRNAs that increased basal [Ca²⁺]_i even transiently caused a reduction in maturation rates, although expression D76A hStim1-YFP+hOrai1-mRFP nearly completely prevented resumption of meiosis. Asterisks above bars represent treatments that significantly reduced GVBD rates (p < 0.05). (B) The effect of coexpression of D76A hStim1-YFP + hOrai1-mRFP on meiotic resumption was investigated as depicted in the flow chart (left), and data are summarized in the bar graph (right). Expression of D76A hStim1-YFP+hOrai1-mRFP in GV oocytes delayed and mostly prevented GVBD under normal [Ca²⁺]_e (p < 0.05). (C) As before, but selected GV-arrested oocytes were transferred to low-[Ca²⁺]_e medium (0.4 mM), which partly rescued the arrest caused by expression of D76A hStim1-YFP+hOrai1-mRFP. (D) Ca²⁺ traces depicting the effect of lowering [Ca²⁺]_e on basal [Ca²⁺]_i in control and GV-arrested D76A hStim1+hOrai1–expressing oocytes monitored before and 2 h after lowering [Ca²⁺]_e from 1.7 to 0.4 mM; the bold trace represents the mean of the responses. (E) A bar graph was used to summarize in the same group of oocytes the mean ± SEM change in basal [Ca²⁺]_i caused by lowering external [Ca²⁺]_e; p < 0.05.