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. 2013 May 1;24(9):1420–1433. doi: 10.1091/mbc.E12-11-0800

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© 2013 Neisch et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Tethering Conu to the membrane results in increased proliferation and influences cell shape. (A and A′) BrdU incorporation in disks expressing myr-conu under the enGal4 driver in the posterior half of the disk (right of the arrowhead) is increased in the expressing cells compared with wild-type anterior cells. (B–B′′) enGal4/+ was used as a control. Proximal wing cells (indicated by arrowheads in B) normally have high BrdU incorporation, while peripodial cells that overlay the wing blade do not (B), but in disks expressing myr-conu in the posterior compartment (to the right of the arrowheads in A and A′), there is increased BrdU incorporation in both layers (A). (C) Wing imaginal disk cells expressing myr-conu under the dppGal4 driver shorten in height and are extruded apically (arrowhead in C′ and C′′′). Coexpression of GFP^NLS was used to mark the expressing cells. (D) Peripodial and disk-proper cells expressing myr-conu under enGal4 are more cuboidal in shape, and some cells are extruded apically from the epithelium and found between the two cell layers (indicated by arrowheads in D and D′′′).