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. 2013 May 1;24(9):1444–1453. doi: 10.1091/mbc.E12-12-0878

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© 2013 Su et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Different subpopulations of CFT-tubules are defined by different septin complexes. (A) A z-section of an embryo from a Sep1-GFP–expressing Drosophila line immunostained with an anti-amphiphysin (Amph) antibody. White arrows point to CFT-tubules. (B) A z-section of an embryo from a Sep4-GFP–expressing Drosophila line immunostained with an anti-amphiphysin (Amph) antibody and an anti-Pnut antibody. White arrows point to CFT-tubules. (C) Quantification of the number of CFT-tubules that stain uniquely with Pnut or amphiphysin (Amph) and CFT-tubules that possess both markers. Error bars, ±SD. n = 11 embryos. (D) Quantification of the length of different CFT-tubule populations at different stages of cleavage furrow ingression. Error bars, ±SD. n = 11 embryos. (E) Different septin-GFP–expressing embryos stained with an anti-amphiphysin (Amph) antibody that define CFT-tubules that have different septin and amphiphysin subdomains. White arrows point to CFT-tubules. Bar, 5 μm.