TABLE 4:

Statistical analysis of YFP-tagged and endogenous acetyl-lysine regulatory factor recruitment to transgene arrays in U2OS and HeLa cells using unpaired t test.

	U2OS 2-6-3 area p values
	Ch-Sp100A vs. Cherry	Ch-Sp100A vs. Ch-Sp100B	Ch-Sp100A vs. Ch-Sp100A (inactive)	Cherry vs. Ch-Sp100A (inactive)	Cherry vs. Ch-Sp100B	Ch-Sp100B vs. Ch-Sp100A (inactive)	Cherry, n	Ch-Sp100A, n	Ch-Sp100B, n	Ch-Sp100A (inactive), n
YFP-GCN5	<0.0001	<0.0001	<0.0001	0.0150	0.6624	0.1459	18	17	15	23
YFP-PCAF	0.0004	<0.0001	<0.0001	0.2320	0.2044	0.7672	16	18	16	30
YFP-p300	0.0036	<0.0001	<0.0001	0.0021	<0.0001	0.3817	15	16	17	28
YFP-BRD2	0.0677	<0.0001	<0.0001	<0.0001	<0.0001	0.9247	19	17	19	24
YFP-BRD4	0.0184	<0.0001	<0.0001	<0.0001	0.0003	0.8920	18	18	14	25
	HeLa HI 1-1 area p values
	Ch-Sp100A vs. Cherry	Ch-Sp100A vs. Ch-Sp100B	Ch-Sp100A vs. Ch-Sp100A (inactive)	Cherry vs. Ch-Sp100A (inactive)	Cherry vs. Ch-Sp100B	Ch-Sp100B vs. Ch-Sp100A (inactive)	Cherry, n	Ch-Sp100A, n	Ch-Sp100B, n	Ch-Sp100A (inactive), n
YFP-GCN5	<0.0001	<0.0001	<0.0001	0.1525	0.0102	<0.0001	14	24	23	19
YFP-PCAF	<0.0001	<0.0001	<0.0001	<0.0001	0.3986	<0.0001	18	22	23	19
YFP-p300	<0.0001	<0.0001	0.0002	0.0006	0.0103	<0.0001	15	25	22	19
YFP-BRD2	<0.0001	<0.0001	<0.0001	0.0514	0.0159	<0.0001	16	26	21	20
YFP-BRD4	<0.0001	<0.0001	<0.0001	0.0291	0.0003	<0.0001	14	26	22	20
	U2OS 2-6-3 area p values
	YFP-Sp100A vs. YFP		YFP-Sp100A vs. YFP-Sp100B		YFP vs. YFP-Sp100B		YFP, n		YFP-Sp100A, n	YFP-Sp100B, n
Anti-BRD4	0.0693		<0.0001		<0.0001		22		24	22
Anti-H4AcK5	0.2342		<0.0001		<0.0001		27		27	25
	HeLa HI 1-1 area p values
	YFP-Sp100A vs. YFP		YFP-Sp100A vs. YFP-Sp100B		YFP vs. YFP-Sp100B		YFP, n		YFP-Sp100A, n	YFP-Sp100B, n
Anti-BRD4	<0.0001		<0.0001		0.0084		20		24	24
Anti-H4AcK5	<0.0001		<0.0001		0.0004		21		24	24