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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Nat Commun. 2012;3:1310. doi: 10.1038/ncomms2306

Figure 3.

Figure 3

PfHsp110c is essential and is required for surviving febrile temperatures. (a) Asynchronous PfHsp110c-RFA parasite clones, 1F9 and 2D8, were grown with or without 10 µM TMP and their growth was monitored over 5 days via flow cytometry. Data are fit to an exponential growth equation and are represented as mean ± S.E.M. (n=3). (b) Asynchronous PfHsp110c-RFA parasite clones, 1F9 and 2D8, were incubated in different TMP concentrations and their growth was monitored after 3 days by flow cytometry. Data are fit to a dose-response equation and are represented as mean ± S.E.M. (n=3). (c) Asynchronous PfHsp110c-RFA parasite clones, 1F9 and 2D8, were subjected to heat shock using the protocol shown, permissive conditions were restored and their growth was monitored over 3 days by flow cytometry. Data are fit to an exponential growth equation and are represented as mean ± S.E.M. (n=3). For each experiment, parasites were subcultured with fresh erythrocytes at day 3 and parasitemia is calculated accounting for culture dilution. Media were changed every day.