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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Nat Commun. 2012;3:1310. doi: 10.1038/ncomms2306

Figure 4.

Figure 4

Function of PfHsp110c-RFA is controlled by TMP. (a, b, c) PfHsp110c-RFA parasites were incubated at 37°C or 40°C with or without 10 µM TMP for 6 hours (indicated at top). PfHsp110c-RFA (using monoclonal αGFP JL8) and PfHsp70 (using polyclonal αHsp70) were detected by immunoblotting after SDS-PAGE. (a) total cell lysates, (b) immunoprecipitation of PfHsp70 using polyclonal αHsp70, (c) immunoprecipitation of PfHsp110c-RFA using monoclonal αGFP 3E6. Parasite clones 1F9 and 2D8 are indicated at the top.