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. Author manuscript; available in PMC: 2013 Apr 29.
Published in final edited form as: Nat Commun. 2012;3:1310. doi: 10.1038/ncomms2306

Figure 5.

Figure 5

The knockdown phenotype is specific and rescued only by PfHsp110c. (a) Parasites were complemented with PfHsp110c-tagRFP-T or tagRFP-T. Asynchronous complemented parasites were incubated at 40°C for 6 hours with or without 10 µM TMP as in Fig. 3c. Their recovery was monitored over 5 days by flow cytometry. Data was fit to an exponential growth equation and are represented as mean ± S.E.M. (n=3). (b) Parasites were complemented with PfHsp110c-tagRFP-T, human Hsp105α-tagRFP-T, yeast Sse1p-tagRFP-T, Sse2p-tagRFP-T or tagRFP-T. Asynchronous complemented parasites were incubated at 40°C for 6 hours without TMP. Their recovery post-heat shock after 5 days was normalized to that of complemented PfHsp110c-RFA parasites that were heat shocked in the presence of 10 µM TMP. Parasitemia was monitored daily via flow cytometry Data are represented as mean ± S.E.M. (n=3). (c) Parasites were incubated at 40°C for 6 hours without TMP with or without 100 nM Thioflavin T (ThT). Their recovery after 5 days was normalized to parasites incubated at 40°C for 6 hours with 10 µM TMP and with or without 100 nM ThT. Data are represented as mean ± S.E.M. (n=3).