Figure 6.
PfHsp110c prevents the aggregation of Asn-rich protein during febrile episodes. Live fluorescence images of PfHsp110c-RFA parasite lines expressing (a, b) the Asn repeat-rich parasite protein, PFI1155w or (c, d) the prion-forming domain from the yeast protein, Sup35 (aa1-125) fused to tagRFP-T (Sup35-tagRFP-T), incubated at 40°C for 6 hours with (a, c) or without (b, d) 10 µM TMP. The arrowheads indicate fluorescent foci of PFI1155w-tagRFP-T (b) and Sup35-tagRFP-T (d). Scale bar represents 2 µm. Images from left to right are phase, DAPI, GFP, RFP and GFP-RFP merge.