Fig. 2.

sox10 function is required for oligodendrocyte differentiation. A–F: Lateral views of spinal cords, dorsal up, showing plp1a, cldnk, and mbp RNA expression in 4.5-dpf larvae. Brackets mark spinal cord (SC) and notochord (NC). G, H: Transverse sections showing MBP expression (red) and anti-acetylated tubulin labeling (blue) at 6 dpf detected by immunocytochemistry. Arrows indicate prominent MBP deposition surrounding Mauthner axons in wild-type larva. I: Quantification of plp1a⁺ and cldnk⁺ cells. Graphs show dorsal, ventral, and total spinal cord cells that express these genes. Error bars represent the SEM. Scale bar, 24 μM.