Figure 2. Extracellular bovine chymosin (CHY) production by A. oryzae autophagy gene disruptants.

Approximately 2×10⁵ conidia of each strain were inoculated into 20 ml 5×DPY medium (pH 5.5) and the CHY activity in the culture supernatant was measured daily after 3–6 days of growth at 30°C. Three experiments were performed, and the values of the average and standard deviations are represented (*p<0.01, Student’s t test).