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. 2013 Apr 29;8(4):e62718. doi: 10.1371/journal.pone.0062718

Figure 6. BSMC expression of RIG-I and MDA5 is upregulated upon RV1B infection.

Figure 6

Infection with increasing doses (MOI) of RV1B for 24 h upregulated BSMC mRNA expression of RIG-I (A) and MDA5 (D) and the expression of both RLRs were further enhanced 48 h post-infection (B, E). Chloroquine (10 µg/ml) partly reduced the effect of RV1B on both RIG-I and MDA5 mRNA expression, especially after 48 h (C, F). RIG-I protein (G) was upregulated at 24 h and more enhanced at the infection dose of 0.1 MOI 48 h post-infection. MDA5 protein (H) was upregulated 48 h post-infection only. Chloroquine reduced RV1B-induced effects on both RIG-I (G) and MDA5 (H) protein at 48 h. Data are presented as mean with SEM and n = 6 for 24 h RV1B infection, n = 3 for 48 h infection and n = 2–3 for chloroquine experiments (BSMCs from three individual donors). Representative western blot images of each receptor protein are shown. *p≤0.05 and **p≤0.01 compared to non-infected cells (control).