Figure 2. The number of synapses per neuron is constant in one and two-neuron micronetworks.

(A) Bright-field images of one- and two-neuron micronetworks with patch clamp recording electrodes in place. Scale bars are 40 µm. (B) VGluT1 immunostaining of glutamatergic synapses in microislands containing one or two neurons (white arrows). The lower panels are enlarged images of the indicated rectangular areas D, The number of synapses in one- and two-neuron microislands is indicated: 330±70 synapses in one-neuron islands (n = 9), and 750±120 synapses in two-neuron islands (n = 7). Scale bars are 40 µm in upper panels and 5 µm in lower panels.