Figure 1.

Expression of CSF-1R at protein level. Expression of CSF-1R examined in canine mammary cancer cell lines using a BD FACSCAria II (BD Biosciences, USA) and analyzed with FACS Diva software (BD Biosciences). The experiment was conducted three times. A. Representative overlay histograms of CSF-1R expression in CMT-U27 canine mammary cancer cells (control), and treated with csf-1r specific siRNA and non-coding siRNA. The overlay histograms were created using Flowing Software (Turku University, Finland), www.flowingsoftware.com. B. Representative overlay histograms of CSF-1R expression in CMT-U27 canine mammary cancer cells (control), and treated with CSF-1 at the dose of 25, 50 and 100 ng/ml. The overlay histograms were created using Flowing Software (Turku University, Finland), www.flowingsoftware.com. C. Representative graphs of the number of CSF-1R expressing cells (CMT-U27) obtained with FACS Aria II (Becton Dickinson). The number of cells which express CSF-1R is significantly decreased after the csf-1r specific siRNA treatment and significantly increased after treatment with CSF-1 at the dose of 25, 50 and 100 ng/ml (no significant differences between these doses have been observed). Treatment of cells with non-coding siRNA did not cause any effect. Error bars refer to S.D. p<0.001 was marked as ***. One-way ANOVA followed by Tukey HSD post-hoc test were applied. D. Representative graphs of the mean fluorescence related to CSF-1R expression in CMT-U27 cell line obtained with FACS Aria II (Becton Dickinson). The mean expression of CSF-1R is significantly decreased after the csf-1r specific siRNA treatment and significantly increased after treatment with CSF-1 at the dose of 25, 50 and 100 ng/ml (no significant differences between these doses have been observed). Treatment of cells with non-coding siRNA did not cause any effect. Error bars refer to S.D. p<0.05 was marked as *, and p<0.001 was marked as ***. One-way ANOVA followed by Tukey HSD post-hoc test were applied.