Figure 4. Phosphorylated ERK1/2 (p-ERK1/2) expression in male and female cerebral arteries after organ culture (OC).

(A) Representative images of p-ERK1/2 in the medial layer (ML) of human cerebral arteries before (0 h OC) and after organ culture (48 h OC). Similar expression of p-ERK1/2 was observed in male arteries, while an increased immunoreactivity was observed in female arteries after organ culture. (B) Fluorescence intensity measurement (arbitrary units, AU) of p-ERK1/2 immunohistochemical staining in the ML.