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. 2013 Apr 29;8(4):e62125. doi: 10.1371/journal.pone.0062125

Figure 2. Stable expression of the mutant U5-200kD protein results in growth defects.

Figure 2

A) Growth defects in the Ponasterone A inducible, stably transfected mutant U5-200kD clones. The uninduced and induced 293/EcR cells show a normal, wild type growth rate, indicating that the Ponasterone A had no effect on growth rate. p<0.001. B) Detection of the mutant U5-200kD gene in SMC10 cells using mutant specific primers. The mutant specific primers detected the mutant U5-200kD mRNA only in the SMC10 uninduced (lane 7 lower band) and induced (lane 9 lower band) cells. The top bands in lanes 3, 5, 7 and 9 are the histone PCR products. Lanes 2, 4, 6 and 8 are minus RT controls showing lack of DNA contamination. Lanes 1 and 10 are the 100 bp markers.