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. 2013 Apr 29;8(4):e62283. doi: 10.1371/journal.pone.0062283

Figure 6. Effects of E Site sequences and downstream pseudoknot stimulator identities on the attenuation activity of M1 hairpin.

Figure 6

(A) The sequences of M1 attenuator hairpin with the mutation site boxed and 5′WT-M1 element with the mutation sites boldly typed.(B) The sequence variation (top), SDS PAGE result of −1 PRF assays (middle) and attenuation efficiencies of M1 attenuator (bottom) of reporter constructs with E site sequence variation. Error bars, s.d.; n = 3. (C) The −1 PRF module set-up (top), SDS PAGE result of −1 PRF assays (middle), and attenuation efficiencies of M1 attenuator (bottom) of reporter constructs with different downstream pseudoknot stimulators. Δ means the attenuator hairpin is deleted. The 0 frame and −1 frame products are designated by filled circles and triangles, respectively. Error bars, s.d.; n = 3.