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. 2013 Apr 29;8(4):e62035. doi: 10.1371/journal.pone.0062035

Figure 2. Differential mRNA expression in CD31+ microvascular fragments.

Figure 2

Microvascular fragments were isolated from mouse tissues using anti-CD31 (PECAM)-coated magnetic beads. Reverse transcription was performed using equal amounts of RNA and expression levels were assessed using real-time quantitative PCR. The graph shows the ratio between expression in microvascular fragments and the remaining tissue fraction for all genes included in the study. Gapdh and an established endothelial marker, Tie2, were included for comparison. Error bars represent SEM (n = 4, P<0.005 in all cases using Student’s t-test).